Zaretsky et al. used iPRECIO® pumps to inject 100nl of bicuculline solution four times over 2 days. Telemetry recordings showed 4 distinct injections. Injections number 3 and 4 were obviously like injections 1 and 2 but blurred by the animal’s intrinsic activity before and during darkness.



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Related Publications

Zaretsky D.V., Zaretskaia M.V., Durant P.J., Rusyniak D.E.
The use of microinfusion pump to perform intrahypothalamic injections in conscious rats.
Neuroscience 2012, New Orleans, USA., October 13 th - 17 2012
http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2964&sKey=87d8b951-316f-466a-9eb7-4b154d0bbd2c&cKey=b4b8338f-9bd2-44e2-bcf4-6e05a36cbbcb&mKey

Zaretsky DV, Zaretskaia MV, Rusyniak DE, Dimicco JA.
Stress-free microinjections in conscious rats
J Neurosci Methods. 2011 Aug 15;199(2):199-207. Epub 2011 May 11
http://www.ncbi.nlm.nih.gov/pubmed/21600924