Tabular highlights gives a high level view of how iPRECIO pumps were used/programmed in selected scientific publications in Applications Examples eBook. For more detailed view, refer eBook and publications . In e-book for example, Research Application 1 (RA1) on page 5 & 6 of eBook is on row 1 of Tabular highlights – Chavan et al. The protocol is once a day infusion. RA2 on pages 7 & 8 (row 2 Kroon et al) has a 3 day stop prior to start of infusion of Nicotinic acid with the animal in the metabolic cage all of the time of the infusion protocol.

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Research Application (RA) 1 - 6  GLP Studies with iPRECIO Pumps  Toxicology with iPRECIO Pumps  Application Examples (AP) 1 - 5  Webinar (WE)  Recently in the news

Research Application (RA)
Author, doi or www.			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
RA 1 Chavan et al. [doi:10.1038/ncomms10580]			pgs 5-6
1 week recovery	Pumps programmed to infuse saline (2ul/hr) or ßOHB (2ul/hr) or sodium pyruvate (5ul/hr) or coconut oil (5ul/hr) prior to meal time (6h, ZT22-ZT) in Restricted Food (RF)	No	Yes	Yes
SC		< 3 weeks
Biological sciences-Neuroscience-Biochemistry Mice + SMP-300 iPRECIO Pump
RA 2 Kroon et al. [doi:10.1194/jlr.M058149]			pgs 7-8
Pump programmed to stop for 3 days	Nicotinic Acid (NiAc) or saline 5 days of continuous or intermittent (12 hr ON/OFF cycles, infusion on 13:00h)	Yes	Yes	Yes
SC		5 or 11 days infusion + 2 days recovery
Adipose tissue-diabetes-drug therapy-Lipoysis and fatty acid metabolism-GPR109A-Niacin-Tachyphylaxis Zucker rat + SMP-200 Pump
RA 3 Suehiro et al. [doi:10.1038/srep04972]			pgs 9-10
No	PKA and ATP - solution change every 2 days, 1ul/hour	Yes	No	No
Intrathecal		14 days
Neural-stem cells-spinal cord injury, rat + SMP-200 Pump
RA 4 Mitchell et al. Developments in LifeSciences Vol. 14 No. 4 < link >			pgs 11-12
1ul/hr saline for 1 week for full recovery	Test articles infusing at 30ul/hr for 72 hours after 1ul/hr saline recovery after catherization and pump implantation	Yes	No	No
Intrathecal		10 days
Regulatory request, CNS end points (modified Irwin assessment), rat + SMP-200 Pump
RA 5 Asemu et al. [doi:10.1152/ajpheart.00674.2012]			pg 13
No	Aldosterone (15% ethanol 50% DMSO and 35% water) was continuous infused	Yes	No	No
IV jugular		14 weeks
Cardiac, diastolic dysfunction, heart failure, metabolism, dog + SMP-200 Pump
RA 6 Thisgaard et al. [doi:10.7150/thno.15898]			pg 14
No	Isotone saline, 0.1 mM MTX, then 0.3µg/ml 125I-UdR, 127I-UdR was continuously infused	Yes	No	No
Brain infusion		10 days
Glioblastoma, Auger-electron therapy, convection delivery, [125I]5-Iodo-2'-deoxyuridine, temozolomide, SMP-200 Pump

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GLP Studies with iPRECIO Pumps (page 15 eBook)
Author			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
Patten et al.			pg 15
1ul/hr artificial CSF	Treament period 30ul/hour for 72 hours	No	Yes	Yes
Intrathecal		3 days for TA
CNS (modified Irwin assessment), rat + SMP-200 pump
Perron et al.			pg 15
No	Flow-rate and dosing validation studies on the bench	No	Yes	Yes
in-vitro		< 7 days
GLP validation
Ringer et al.			pg 15
Saline when not infusing test article	2 hour baseline, 2 hour infusion (Milrinone or vehicle)	No	Yes	Yes
Right jugular vein		24 hours
GLP validation, jacktet, ambulatory, cardiovascular, Dog + iPRECIO Dual

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Toxicology with iPRECIO Pumps (page 15 eBook)
Author, doi:			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
Tsuboi et al. [doi:10.2131/fts.3.1]			pg 15
No but full study saline infusion	Saline infusion for 4 weeks and 13 weeks, 2 or 2.5ul/hour	Yes	No	No
IV jugular		4 or 13 weeks
Infusion pump, implantation, rat, iPRECIO, Physiological condition, SMP-200 pump

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Application Examples (AP) (page 16 eBook)
Author, doi/www			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
AP1 Tan et al. [doi:10.3389/fphar.2011.00044]			pg 16
3 days pump "OFF" (0.2ul/hour) , for control cardiovascular parameters	5-HT dose response with control period	No	Yes	Yes
SC		30 days
drug delivery, minipump, osmotic pumps, implantable pump, dose response, quantitative pharmacology, preclinical, telemetry, rat + SMP-200 pump
AP 2 Tan et al. [doi:10.3389/fphar.2011.00044]			pg 16
11 day recovery period	Saline, dobutamine and verapamil infusions	No	Yes	Yes
IV		30 days
drug delivery, minipump, osmotic pumps, implantable pump, dose response, quantitative pharmacology, preclinical, telemetry, rat + SMP-200 pump
AP 3 Zaretsky et al. < link >			pg 16
5 day recovery but no flow	BMI (0.1 mM) 100 nl injections	No	Yes	Yes
Intra-hypothalamic		several days
hypothalamas, cardiovasular, micro injections, rat + SMP-200 pump
AP 4 Kuroki et al. [doi:10.1152/ajpheart.00922.2013]			pg 16
7 to 10 days recovery then 4 days control period	5ul/hour continous infusion of Ang II. Comparison made with Alzet Pump and Havard Sringe pump	Yes	No	No
SC administration		2 weeks Ang II infusion
salt-sensitive hypertension; angiotensin II-salt hypertension; Alzet salt-sensitive hypertension; minipump; iPrecio pump; plasma angiotensin II
AP 5 Gey et al. [doi:10.1016/j.nbd.2016.03.012]			*Not in eBook
No RCV	0.2 ul/hr continuos infusion. Stability of vigabatrin was tested and confirmed for 5 weeks	Yes	No	No
Bilateral infusion into STN or anterior SNr		3 or 4 week
minipump; iPrecio pump; plasma angiotensin II, Rats, SMP-200

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Webinar (WE)
Author, www or doi			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
WE1 Schnell < link >			pgs 17-18
Saline infusion for tumor size to grow to a certain size (12th day)	On day 21 (tumor reached certain size), infusion of Compound A for 22 days. Behaviour and weight gain not impaired versus control without subcutaneous pump	Yes	No	No
Intra-cranial infusion		22 days
Cancer, orthotopic, glioblastoma, bioluminecscence, Nude rats, SMP-200
WE2 Doyle < link > (Henry et al. [doi:10.1210/en.2014-1825])			pgs 17-18
7 days recovery Saline, 5 days baseline Saline, 5 day TA or 10 day TA	Vitamin B12 Conjugation of Peptide-YY3–36 or Native Peptide-YY3–36 or saline 5 pulses per day; three 1 hour pulses of 10 nmol.kg-1.h-1 (20 μl.h-1) with three hours between pulses and two 1 hour pulses of 5 nmol.kg-1.h-1 (10 μl.h-1) with five hours between pulses	No	Yes	Yes
SC		5 or 10 days
endocrinology, vitamin B12, BBB, reduction of food intake, peptides, rats, SMP-200 pumps

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Recently in the news
Author, www or doi			pg(s) number of eBook
Refillability of Pump		How pump programmed?
KVO/RCV	Vehicle and Drug(s)	Continuous	Intermittent or bolus	Circadian / Timed release
Adminstration Site		Duration
Key words
Dey et al. [doi: 10.1161/CIRCRESAHA.118.312708]			pg 25
No	Daily bolus of Isoproterenol (ISO) was administered via implantation of a programmable iPRECIO® Pump in the peritoneal cavity. The iPRECIO pump enables reliable, timed delivery of pharmacological agents. The pump was programmed for 1 hour delivery of Isoproterenol at 30μl/hr, total dose of 2mg/kg/day, once a day at the same time (1 PM).	No	Yes	Yes
IP		3 to 5 weeks
ventricular fibrillation, phosphorylation, proteomics, heart failure, reactive oxygen species, mitochondria, 300g hartley guinea pig, SMP-200
Steplewski et al. [doi: 10.1002/jor.23369]			*New: Not in eBook.
No	The delivery of the ACA and P-ACA started immediately after surgery and continued for 8 weeks at 1ul/h.	Yes	No	No
Injured knee joint		8 weeks
joint contracture, posterior capsule collagen, collagen fibrils, arthrofibrosis, antibody, New Zealand White Rabbits, 8-12mths, SMP-200